Surface Species in Graphene Liquid Cells for Transmission Electron Microscopy

Transmission Electron Microscopy of Lipid Vesicles in Liquid Cells

Liquid cell transmission electron microscopy (TEM) shows promise for enabling nanometer-scale imaging of fluid-hydrated biological material by enclosing specimens within windowed devices [1]. However, the thickness of the liquid cell reduces the signal-to-noise ratios of acquired images, a particular problem for researchers imaging biological specimens. Thus investigators typically image biolog...

Graphene Liquid Enclosure for Single-Molecule Analysis of Membrane Proteins in Whole Cells Using Electron Microscopy.

Membrane proteins govern many important functions in cells via dynamic oligomerization into active complexes. However, analytical methods to study their distribution and functional state in relation to the cellular structure are currently limited. Here, we introduce a technique for studying single-membrane proteins within their native context of the intact plasma membrane. SKBR3 breast cancer c...

Direct Observation of Wet Biological Samples by Graphene Liquid Cell Transmission Electron Microscopy.

Recent development of liquid phase transmission electron microscopy (TEM) enables the study of specimens in wet ambient conditions within a liquid cell; however, direct structural observation of biological samples in their native solution using TEM is challenging since low-mass biomaterials embedded in a thick liquid layer of the host cell demonstrate low contrast. Furthermore, the integrity of...

Writing silica structures in liquid with scanning transmission electron microscopy.

Silica nanoparticles are imaged in solution with scanning transmission electron microscopy (STEM) using a liquid cell with silicon nitride (SiN) membrane windows. The STEM images reveal that silica structures are deposited in well-defined patches on the upper SiN membranes upon electron beam irradiation. The thickness of the deposits is linear with the applied electron dose. Scanning electron m...

Scanning Transmission Electron Microscopy of Metal- Graphene Interaction